LDH

Intended use

Biostep Diagnostic’s liquizyme LDH reagent is intended for the invitro quantitative, diagnostic determination of LDH in human serum on both automated and manual systems.

Assay principle

LDH catalyzes the reaction between pyruvate and NADH to produce NAD+ and L-Lactate:

Pyruvate + NADH + H L- Lactate + NAD

The initial rate of the NADH oxidation is directly proportional to the catalytic LDH activity. It is determined by measuring the decrease in absorbance at 340 nm.