GOT (kinetic)

Intended Use

Biostep Diagnostic’s AST reagent is intended for the invitro quantitative, diagnostic determination of AST in human serum on both automated and manual systems.

Background

The enzyme aspartate aminotransferase (AST) is widely distributed in erythrocytes and tissues, principally heart, liver, muscle, and kidney. Elevated serum levels are found in diseases involving these tissues such as myocardial infarction, viral hepatitis and muscular dystrophy. Following myocardial infarction, serum AST is elevated and reaches a peak two days after onset. Two isoenzymes of AST have been detected, cytoplasmic and mitochondrial. Only the cytoplasmic isoenzyme occurs in normal serum, while the mitochondrial, together with the cytoplasmic isoenzyme, has been detected in the sera of patients with coronary and hepatobiliary diseases.