G-6PDH (UV- kinetic)

Intended use

Biostep G-6-PDH reagent is intended for the in-vitro quantitative UV diagnostic estimation of G-6-PDH in RBC’s.

Assay principle

G6PDH in the RBC’s is released by a lysing agent present in the reagent. The G6PDH released catalyzes the oxidation of Glucose-6-phosphate with the reduction of NADP to NADPH. The rate of reduction of NADP to NADPH is measured as an increase in absorbance which is proportional to the G6PDH activity in the sample.